Difference between revisions of "NifS"

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|colspan="2" | '''Genetic context''' <br/> [[Image:nifS_context.gif]]
 
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nifS_2849569_2850756_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:nifS_expression.png|500px]]
 
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Revision as of 10:05, 20 April 2012

  • Description: cysteine desulfurase, required for NAD biosynthesis

Gene name nifS
Synonyms iscS
Essential no
Product cysteine desulfurase
Function NAD biosynthesis
MW, pI 43 kDa, 6.425
Gene length, protein length 1185 bp, 395 aa
Immediate neighbours nadB, nadR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NifS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NifS expression.png
























Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

NadR regulon

The gene

Basic information

  • Locus tag: BSU27880

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine (according to Swiss-Prot)
  • Protein family: NifS/iscS subfamily (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed in the presence of nicotinic acid (NadR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References