Difference between revisions of "GanA"

From SubtiWiki
Jump to: navigation, search
m (moved LacA to GanA: 22893383)
Line 4: Line 4:
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''lacA''
+
|''ganA''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yvfN ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yvfN, lacA ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
Line 14: Line 14:
 
|style="background:#ABCDEF;" align="center"|'''Function''' || galactan utilization
 
|style="background:#ABCDEF;" align="center"|'''Function''' || galactan utilization
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU34130 lacA]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU34130 ganA]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 79 kDa, 5.704   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 79 kDa, 5.704   
Line 20: Line 20:
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2061 bp, 687 aa  
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2061 bp, 687 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvfO]]'', ''[[yvfM]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ganB]]'', ''[[ganQ]]''
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15418&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15418&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
Line 32: Line 32:
  
 
__TOC__
 
__TOC__
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
  
 
<br/><br/>
 
<br/><br/>
Line 55: Line 52:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
* a ''[[cycB]]-[[ganP]]-[[ganQ]]-[[ganA]]-[[ganB]]'' mutant is impaired in the utilization of galactan {{PubMed|22893383}}
  
 
=== Database entries ===
 
=== Database entries ===
Line 63: Line 61:
  
 
=== Additional information===
 
=== Additional information===
 
 
  
  
Line 71: Line 67:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides (according to Swiss-Prot)
+
* '''Catalyzed reaction/ biological activity:'''  
 +
** degradation of galactotriose to galactose {{PubMed|22893383}}
  
 
* '''Protein family:''' glycosyl hydrolase 42 family (according to Swiss-Prot)
 
* '''Protein family:''' glycosyl hydrolase 42 family (according to Swiss-Prot)
Line 107: Line 104:
  
 
=Expression and regulation=
 
=Expression and regulation=
* '''Operon:''' ''[[cycB]]-[[yvfL]]-[[yvfM]]-[[lacA]]-[[yvfO]]'' {{PubMed|9287030}}  
+
* '''Operon:''' ''[[cycB]]-[[ganP]]-[[ganQ]]-[[ganA]]-[[ganB]]'' {{PubMed|9287030}}  
  
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ganA_3503020_3505083_-1 lacA] {{PubMed|22383849}}
+
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ganA_3503020_3505083_-1 ganA] {{PubMed|22383849}}
  
 
* '''Sigma factor:'''  
 
* '''Sigma factor:'''  
Line 140: Line 137:
 
=References=
 
=References=
  
<pubmed>2104611,9287030,17056685 , 22517742</pubmed>
+
<pubmed>2104611,9287030,17056685 , 22517742 22893383</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 13:53, 16 August 2012

  • Description: beta-galactosidase

Gene name ganA
Synonyms yvfN, lacA
Essential no
Product beta-galactosidase
Function galactan utilization
Gene expression levels in SubtiExpress: ganA
MW, pI 79 kDa, 5.704
Gene length, protein length 2061 bp, 687 aa
Immediate neighbours ganB, ganQ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LacA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
LacA expression.png















Categories containing this gene/protein

utilization of specific carbon sources, phosphoproteins

This gene is a member of the following regulons

LacR regulon

The gene

Basic information

  • Locus tag: BSU34130

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • degradation of galactotriose to galactose PubMed
  • Protein family: glycosyl hydrolase 42 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on Arg-387 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:GP1181 (aphA3) available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References