Difference between revisions of "CheD"

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* '''Mutant:'''
 
* '''Mutant:'''
 
** 1A861 ( ''cheD''::''cat''), {{PubMed|7893679}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A861&Search=1A861 BGSC]
 
** 1A861 ( ''cheD''::''cat''), {{PubMed|7893679}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A861&Search=1A861 BGSC]
 +
** 1A863 ( ''cheD''::''cat''), {{PubMed|7893679}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A863&Search=1A863 BGSC]
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 09:01, 19 September 2012

  • Description: protein deaminase, required for methylation of methyl-accepting chemotaxis proteins by CheR, enhances phosphatase activity of CheC, required for full McpC activity

Gene name cheD
Synonyms ylxK
Essential no
Product protein deaminase, CheC activity modulator
Function motility and chemotaxis
Gene expression levels in SubtiExpress: cheD
Interactions involving this protein in SubtInteract: CheD
MW, pI 17 kDa, 9.347
Gene length, protein length 498 bp, 166 aa
Immediate neighbours cheC, sigD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CheD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CheD expression.png















Categories containing this gene/protein

protein modification, motility and chemotaxis

This gene is a member of the following regulons

CodY regulon, SigD regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU16460

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • Protein L-glutamine + H2O = protein L-glutamate + NH3 (according to Swiss-Prot)
    • deaminates Gln-586, Gln-593, and Gln594 in McpA PubMed
    • deaminates Gln-609 in McpC PubMed
  • Protein family: cheD family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:
    • in minimal medium, CheD is present with 1,200 +/- 250 molecules per cell PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Travis J Muff, George W Ordal
The diverse CheC-type phosphatases: chemotaxis and beyond.
Mol Microbiol: 2008, 70(5);1054-61
[PubMed:18990184] [WorldCat.org] [DOI] (I p)

Christopher V Rao, George D Glekas, George W Ordal
The three adaptation systems of Bacillus subtilis chemotaxis.
Trends Microbiol: 2008, 16(10);480-7
[PubMed:18774298] [WorldCat.org] [DOI] (P p)

Original publications