MetC

• Description: cystathionine beta-lyase
  
  

Gene name	metC
Synonyms	yjcJ
Essential	no
Product	cystathionine beta-lyase
Function	biosynthesis of methionine
Gene expression levels in SubtiExpress: metC
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	42 kDa, 5.757
Gene length, protein length	1170 bp, 390 aa
Immediate neighbours	metI, yjcK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

S-box

The gene

Basic information

• Locus tag: BSU11880

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-cystathionine + H₂O = L-homocysteine + NH₃ + pyruvate (according to Swiss-Prot)

• Protein family: trans-sulfuration enzymes family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O31632

• KEGG entry: [3]

• E.C. number: 4.4.1.8

Expression and regulation

• Operon: metI-metC PubMed

• Expression browser: metC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • induced by methionine starvation (S-box) PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A944 ( metC::kan), PubMed, available at BGSC
  • 1A940 ( metC::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: