PhrC

• Description: response regulator aspartate phosphatase (RapC) regulator / competence and sporulation stimulating factor (CSF)
  
  

Gene name	phrC
Synonyms
Essential	no
Product	phosphatase (RapC) regulator / competence and sporulation stimulating factor (CSF)
Function	control of ComA activity
Gene expression levels in SubtiExpress: phrC
Interactions involving this protein in SubtInteract: PhrC
MW, pI	4 kDa, 8.16
Gene length, protein length	120 bp, 40 aa
Immediate neighbours	rapC, yczM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, transcription factors and their control, quorum sensing, short peptides

This gene is a member of the following regulons

CcpA regulon, CodY regulon, ComA regulon, SigH regulon

The gene

Basic information

• Locus tag: BSU03780

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds to RapC, this results in the inability of RapC to interact with ComA PubMed

• Protein family: phr family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RapC-PhrC PubMed
  • RapJ-PhrC PubMed

• Localization:

Database entries

• Structure:
  • 4GYO (complex RapJ-PhrC) PubMed

• UniProt: P94416

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • rapC-phrC PubMed
  • phrC PubMed

• Expression browser: phrC PubMed

• Sigma factor:
  • rapC: SigA PubMed
  • phrC: SigH PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • repressed by glucose (4.5-fold) (CcpA) PubMed
  • expressed at high cell density (ComA) PubMed

• Regulatory mechanism:
  • ComA: transcription activation (ComA) PubMed
  • CodY: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Elizabeth Anne Shank, Roberto Kolter
Extracellular signaling and multicellularity in Bacillus subtilis.
Curr Opin Microbiol: 2011, 14(6);741-7
[PubMed:22024380] [WorldCat.org] [DOI] (I p)