Difference between revisions of "Mfd"
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the (A)BC excinuclease to the RNA damage site | the (A)BC excinuclease to the RNA damage site | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00550 mfd] |
[[RNA polymerase]] stalled at a nucleotide lesion and directing | [[RNA polymerase]] stalled at a nucleotide lesion and directing | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fin]]'', ''[[spoVT]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fin]]'', ''[[spoVT]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00550 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00550 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00550 Advanced_DNA] |
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|colspan="2" | '''Genetic context''' <br/> [[Image:mfd_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:mfd_context.gif]] | ||
Revision as of 11:55, 13 May 2013
- Description: transcription-repair coupling factor
| Gene name | mfd |
| Synonyms | |
| Essential | no |
| Product | transcription-repair coupling factor |
| Function | promotes strand-specific DNA repair by displacing
RNA polymerase stalled at a nucleotide lesion and directing the (A)BC excinuclease to the RNA damage site |
| Gene expression levels in SubtiExpress: mfd
RNA polymerase stalled at a nucleotide lesion and directing the (A)BC excinuclease to the RNA damage site | |
| Interactions involving this protein in SubtInteract: Mfd | |
| MW, pI | 133 kDa, 5.367 |
| Gene length, protein length | 3531 bp, 1177 aa |
| Immediate neighbours | fin, spoVT |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
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Contents
Categories containing this gene/protein
DNA repair/ recombination, transcription
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00550
Phenotypes of a mutant
In an mfd knock-out, the cell's ability to accumulate adaptive mutations in stationary phase is depressed. PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- promotes strand-specific DNA repair by displacing RNA polymerase stalled at a nucleotide lesion and directing the (A)BC excinuclease to the RNA damage site
- is required for roadblock transcription repression by transcription factors with binding sites downstream of the promoter (as for CcpA PubMed and CodY PubMed)
- Protein family:
- Paralogous protein(s): RecG
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P37474
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP1167 (del ermC), available in Stülke lab
- Expression vector:
- lacZ fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original Articles
Additional publications: PubMed
