Difference between revisions of "CshB"
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|style="background:#ABCDEF;" align="center"|'''Function''' || RNA helicase | |style="background:#ABCDEF;" align="center"|'''Function''' || RNA helicase | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25140 cshB] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CshB CshB] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CshB CshB] | ||
| Line 24: | Line 24: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nfo]]'', ''[[yqfQ]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nfo]]'', ''[[yqfQ]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25140 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25140 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25140 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:yqfR_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:yqfR_context.gif]] | ||
Revision as of 13:19, 13 May 2013
- Description: DEAD-box RNA helicase, important for adaptation to low temperatures
| Gene name | cshB |
| Synonyms | yqfR |
| Essential | no |
| Product | DEAD-box RNA helicase |
| Function | RNA helicase |
| Gene expression levels in SubtiExpress: cshB | |
| Interactions involving this protein in SubtInteract: CshB | |
| MW, pI | 49 kDa, 9.89 |
| Gene length, protein length | 1314 bp, 438 aa |
| Immediate neighbours | nfo, yqfQ |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
DEAD-box RNA helicases, translation
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU25140
Phenotypes of a mutant
- poor growth at low temperatures (16 to 20°C) PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Protein family: DEAD-box RNA helicase
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasma, colocalizes with the ribosomes PubMed
Database entries
- Structure:
- UniProt: P54475
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP1051 (cat), available in Jörg Stülke's lab
- Expression vector:
- for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1064 (spc), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- FLAG-tag construct:
- GP1011 (spc, based on pGP1331), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Mohamed Marahiel, Marburg University, Germany homepage
Your additional remarks
References
Additional publications: PubMed
