CotC

• Description: spore coat protein (outer)
  
  

Gene name	cotC
Synonyms
Essential	no
Product	spore coat protein (outer)
Function	resistance of the spore
Gene expression levels in SubtiExpress: cotC
Interactions involving this protein in SubtInteract: CotC
MW, pI	14 kDa, 9.558
Gene length, protein length	354 bp, 118 aa
Immediate neighbours	ynzK, tatAC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

GerE regulon, SigK regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU17700

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • CotC-CotU PubMed
  • CotE-CotC PubMed

• Localization:
  • outer spore coat, more abundant at the mother cell-distal pole of the forespore PubMed
  • the CotC-CotU complex assembles around the forming spore PubMed

Database entries

• Structure:

• UniProt: P07790

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotC (according to DBTBS)

• Expression browser: cotC PubMed

• Sigma factor: SigK PubMed

• Regulation:
  • expressed late during sporulation in the mother cell (SigK, GerE, SpoIIID) PubMed

• Regulatory mechanism:
  • GerE: transcription activation PubMed
  • SpoIIID: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1S103 ( cotC::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Application of CotZ for protein surface display

Additional publications: PubMed

Rachele Isticato, Donatella Scotto Di Mase, Emilia M F Mauriello, Maurilio De Felice, Ezio Ricca
Amino terminal fusion of heterologous proteins to CotC increases display efficiencies in the Bacillus subtilis spore system.
Biotechniques: 2007, 42(2);151-2, 154, 156
[PubMed:17373477] [WorldCat.org] [DOI] (P p)