XlyA

• Description: N-acetylmuramoyl-L-alanine amidase
  
  

Gene name	xlyA
Synonyms
Essential	no
Product	N-acetylmuramoyl-L-alanine amidase
Function	PBSX prophage-mediated lysis
Gene expression levels in SubtiExpress: xlyA
MW, pI	31 kDa, 5.342
Gene length, protein length	891 bp, 297 aa
Immediate neighbours	xhlB, spoIISB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover, PBSX prophage

The gene

Basic information

• Locus tag: BSU12810

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s): CwlA, CwlH, XlyB

Extended information on the protein

• Kinetic information:

• Domains:
  • contains a N-acetylglucosamine-polymer-binding LysM domain PubMed
  • contains an amidase_2 domain (like BlyA, CwlA, CwlH, XlyB)

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (no signal peptide) PubMed

Database entries

• Structure: 3HMA PubMed

• UniProt: P39800

• KEGG entry: [2]

• E.C. number: 3.5.1.28

Expression and regulation

• Operon:

• Expression browser: xlyA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Girbe Buist, Anton Steen, Jan Kok, Oscar P Kuipers
LysM, a widely distributed protein motif for binding to (peptido)glycans.
Mol Microbiol: 2008, 68(4);838-47
[PubMed:18430080] [WorldCat.org] [DOI] (I p)