IolA

• Description: methylmalonate-semialdehyde dehydrogenase (acylating)
  
  

Gene name	iolA
Synonyms	mmsA, yxdA
Essential	no
Product	methylmalonate-semialdehyde dehydrogenase (acylating)
Function	myo-inositol catabolism
Gene expression levels in SubtiExpress: iolA
Metabolic function and regulation of this protein in SubtiPathways: iolA
MW, pI	53 kDa, 5.139
Gene length, protein length	1461 bp, 487 aa
Immediate neighbours	iolB, iolR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, IolR regulon

The gene

Basic information

• Locus tag: BSU39760

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: NAD-dependent oxidation of methylmalonate semialdehyde (MMSA) to propionyl-CoA via acylation and deacylation steps

• Protein family: IolA subfamily (according to Swiss-Prot)

• Paralogous protein(s): YfmT, AldY, YcbD, GabD, GbsA, DhaS, PutC, RocA, AldX, YwdH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): Coenzyme A, NAD PubMed

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1T90 PubMed

• UniProt: P42412

• KEGG entry: [3]

• E.C. number: 1.2.1.27

Expression and regulation

• Operon: iolA-iolB-iolC-iolD-iolE-iolF-iolG-iolH-iolI-iolJ PubMed

• Expression browser: iolA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by inositol (IolR) PubMed

• Regulatory mechanism:
  • IolR: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Yasutaro Fujita, University of Fukuyama, Japan
• Ken-ichi Yoshida, Kobe University, Japan

References

Additional publications: PubMed