Difference between revisions of "CysC"

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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15600&redirect=T BSU15600]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cysHP-sat-cysC-ylnDEF.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cysHP-sat-cysC-ylnDEF.html]
Line 96: Line 97:
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU15600&redirect=T BSU15600]
  
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3CR8 3CR8] (from ''Thiobacillus denitrificans'', 46% identity, 62% similarity) {{PubMed|19770499}}
 
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3CR8 3CR8] (from ''Thiobacillus denitrificans'', 46% identity, 62% similarity) {{PubMed|19770499}}

Revision as of 13:40, 2 April 2014

  • Description: adenylyl-sulfate kinase

Gene name cysC
Synonyms ylnC
Essential no
Product adenylyl-sulfate kinase
Function sulfate reduction and activation
Gene expression levels in SubtiExpress: cysC
Metabolic function and regulation of this protein in SubtiPathways:
cysC
MW, pI 22 kDa, 5.083
Gene length, protein length 591 bp, 197 aa
Immediate neighbours sat, ylnD
Sequences Protein DNA DNA_with_flanks
Genetic context
CysC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CysC expression.png




























Categories containing this gene/protein

sulfur metabolism

This gene is a member of the following regulons

CymR regulon, S-box

The gene

Basic information

  • Locus tag: BSU15600

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate (according to Swiss-Prot)
  • Protein family: APS kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3CR8 (from Thiobacillus denitrificans, 46% identity, 62% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
    • repressed in the presence of cysteine (CymR)
    • induced by methionine starvation (S-box) PubMed
  • Regulatory mechanism:
    • S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed
    • CymR: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Isabelle Martin-Verstraete, Institute Pasteur, Paris, France

Your additional remarks

References