AnsB

• Description: L-aspartase
  
  

Gene name	ansB
Synonyms
Essential	no
Product	L-aspartase
Function	aspartate degradation
Gene expression levels in SubtiExpress: ansB
Metabolic function and regulation of this protein in SubtiPathways: ansB
MW, pI	52 kDa, 5.69
Gene length, protein length	1425 bp, 475 aa
Immediate neighbours	mleN, ansA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids, membrane proteins

This gene is a member of the following regulons

AnsR regulon

The gene

Basic information

• Locus tag: BSU23570

Database entries

• BsubCyc: BSU23570

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-aspartate = fumarate + NH₃ (according to Swiss-Prot)

• Protein family: Aspartase subfamily (according to Swiss-Prot)

• Paralogous protein(s): CitG

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU23570

• Structure: 3R6Q (from Bacillus sp. YM55-1, 72% identity, 92% similarity) PubMed

• UniProt: P26899

• KEGG entry: [3]

• E.C. number: 4.3.1.1

Expression and regulation

• Operon:
  • ansA-ansB PubMed
  • ansA-ansB-mleN-mleA PubMed

• Expression browser: ansB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the presence of asparagine (AnsR) PubMed

• Regulatory mechanism:
  • AnsR: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 893 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 118 PubMed

• • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 35 PubMed

Biological materials

• Mutant: GP1153 (del ansAB::ermC) available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed