Difference between revisions of "BirA"
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| − | * '''Description:''' [[trigger enzymes|trigger enzyme]]: transcriptional repressor ([[transcription factors of the | + | * '''Description:''' [[trigger enzymes|trigger enzyme]]: transcriptional repressor ([[transcription factors of the BirA family]]) of the biotin operon / biotin-protein ligase <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] | |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || [[trigger enzymes|trigger enzyme]]: transcriptional repressor ([[transcription factors of the | + | |style="background:#ABCDEF;" align="center"| '''Product''' || [[trigger enzymes|trigger enzyme]]: transcriptional repressor ([[transcription factors of the BirA family]])/ biotin-protein ligase |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of biotin synthesis, addition of biotin to proteins | |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of biotin synthesis, addition of biotin to proteins | ||
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** ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] (according to Swiss-Prot) | ** ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)] (according to Swiss-Prot) | ||
| − | * '''Protein family:''' ([[transcription factors of the | + | * '''Protein family:''' ([[transcription factors of the BirA family]]), pyrroline-5-carboxylate reductase family (according to Swiss-Prot) |
* '''Paralogous protein(s):''' | * '''Paralogous protein(s):''' | ||
Revision as of 13:12, 22 June 2014
- Description: trigger enzyme: transcriptional repressor (transcription factors of the BirA family) of the biotin operon / biotin-protein ligase
| Gene name | birA |
| Synonyms | |
| Essential | yes PubMed |
| Product | trigger enzyme: transcriptional repressor (transcription factors of the BirA family)/ biotin-protein ligase |
| Function | regulation of biotin synthesis, addition of biotin to proteins |
| Gene expression levels in SubtiExpress: birA | |
| Metabolic function and regulation of this protein in SubtiPathways: BirA | |
| MW, pI | 36 kDa, 6.769 |
| Gene length, protein length | 975 bp, 325 aa |
| Immediate neighbours | panB, cca |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biosynthesis of lipids, biosynthesis of cofactors, transcription factors and their control, trigger enzyme, essential genes
This gene is a member of the following regulons
The BirA regulon
The gene
Basic information
- Locus tag: BSU22440
Phenotypes of a mutant
essential PubMed
Database entries
- BsubCyc: BSU22440
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: (transcription factors of the BirA family), pyrroline-5-carboxylate reductase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU22440
- Structure:
- UniProt: P42975
- KEGG entry: [2]
- E.C. number: 6.3.4.15
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
Sarah K Henke, John E Cronan
Successful conversion of the Bacillus subtilis BirA Group II biotin protein ligase into a Group I ligase.
PLoS One: 2014, 9(5);e96757
[PubMed:24816803]
[WorldCat.org]
[DOI]
(I e)
Vandana Chakravartty, John E Cronan
The wing of a winged helix-turn-helix transcription factor organizes the active site of BirA, a bifunctional repressor/ligase.
J Biol Chem: 2013, 288(50);36029-39
[PubMed:24189073]
[WorldCat.org]
[DOI]
(I p)
Ahmed Gaballa, Haike Antelmann, Chris J Hamilton, John D Helmann
Regulation of Bacillus subtilis bacillithiol biosynthesis operons by Spx.
Microbiology (Reading): 2013, 159(Pt 10);2025-2035
[PubMed:23894131]
[WorldCat.org]
[DOI]
(I p)
Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541]
[WorldCat.org]
[DOI]
(I p)
J M Lee, S Zhang, S Saha, S Santa Anna, C Jiang, J Perkins
RNA expression analysis using an antisense Bacillus subtilis genome array.
J Bacteriol: 2001, 183(24);7371-80
[PubMed:11717296]
[WorldCat.org]
[DOI]
(P p)
J B Perkins, S Bower, C L Howitt, R R Yocum, J Pero
Identification and characterization of transcripts from the biotin biosynthetic operon of Bacillus subtilis.
J Bacteriol: 1996, 178(21);6361-5
[PubMed:8892842]
[WorldCat.org]
[DOI]
(P p)
S Bower, J B Perkins, R R Yocum, C L Howitt, P Rahaim, J Pero
Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.
J Bacteriol: 1996, 178(14);4122-30
[PubMed:8763940]
[WorldCat.org]
[DOI]
(P p)
S Bower, J Perkins, R R Yocum, P Serror, A Sorokin, P Rahaim, C L Howitt, N Prasad, S D Ehrlich, J Pero
Cloning and characterization of the Bacillus subtilis birA gene encoding a repressor of the biotin operon.
J Bacteriol: 1995, 177(9);2572-5
[PubMed:7730294]
[WorldCat.org]
[DOI]
(P p)
