DivIB

• Description: cell-division initiation protein (septum formation)
  
  

Gene name	divIB
Synonyms	dds, tms-12
Essential	yes PubMed
Product	cell-division protein
Function	septum formation
Metabolic function and regulation of this protein in SubtiPathways: Cell wall
MW, pI	30 kDa, 9.244
Gene length, protein length	789 bp, 263 aa
Immediate neighbours	murB, ylxW
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell division, sporulation proteins, cell envelope stress proteins (controlled by SigM, W, X, Y), essential genes, membrane proteins

This gene is a member of the following regulons

SigE regulon, SigM regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU15240

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ftsQ family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • PbpB-DivIB-(FtsL-DivIC)2 PubMed, as part of the divisome
  • WalK-DivIB PubMed

• Localization: cell membrane PubMed

Database entries

• Structure: 2ALJ (major extracytoplasmic domain, Geobacillus stearothermophilus)

• UniProt: P16655

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• • murE-mraY-murD-spoVE-murG-murB-divIB-ylxW-ylxX-sbp PubMed
  • spoVE-murG-murB-divIB-ylxW-ylxX-sbp PubMed
  • murG-murB-divIB-ylxW-ylxX-sbp PubMed
  • divIB-ylxW-ylxX-sbp PubMed

• Sigma factor:
  • spoVE: SigE PubMed
  • murG: SigMPubMed
  • divIB: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Susan L Rowland, Kimberly D Wadsworth, Scott A Robson, Carine Robichon, Jon Beckwith, Glenn F King
Evidence from artificial septal targeting and site-directed mutagenesis that residues in the extracytoplasmic β domain of DivIB mediate its interaction with the divisomal transpeptidase PBP 2B.
J Bacteriol: 2010, 192(23);6116-25
[PubMed:20870765] [WorldCat.org] [DOI] (I p)

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)

E J Harry, S L Rowland, M S Malo, R G Wake
Expression of divIB of Bacillus subtilis during vegetative growth.
J Bacteriol: 1994, 176(4);1172-9
[PubMed:8106328] [WorldCat.org] [DOI] (P p)

G Theeragool, A Miyao, K Yamada, T Sato, Y Kobayashi
In vivo expression of the Bacillus subtilis spoVE gene.
J Bacteriol: 1993, 175(13);4071-80
[PubMed:8320223] [WorldCat.org] [DOI] (P p)

L S Thompson, P L Beech, G Real, A O Henriques, E J Harry
Requirement for the cell division protein DivIB in polar cell division and engulfment during sporulation in Bacillus subtilis.
J Bacteriol: 2006, 188(21);7677-85
[PubMed:16936026] [WorldCat.org] [DOI] (P p)

Richard A Daniel, Marie-Françoise Noirot-Gros, Philippe Noirot, Jeff Errington
Multiple interactions between the transmembrane division proteins of Bacillus subtilis and the role of FtsL instability in divisome assembly.
J Bacteriol: 2006, 188(21);7396-404
[PubMed:16936019] [WorldCat.org] [DOI] (P p)

Gonçalo Real, Sabine Autret, Elizabeth J Harry, Jeffery Errington, Adriano O Henriques
Cell division protein DivIB influences the Spo0J/Soj system of chromosome segregation in Bacillus subtilis.
Mol Microbiol: 2005, 55(2);349-67
[PubMed:15659156] [WorldCat.org] [DOI] (P p)

R A Daniel, J Errington
Intrinsic instability of the essential cell division protein FtsL of Bacillus subtilis and a role for DivIB protein in FtsL turnover.
Mol Microbiol: 2000, 36(2);278-89
[PubMed:10792716] [WorldCat.org] [DOI] (P p)

V L Katis, R G Wake
Membrane-bound division proteins DivIB and DivIC of Bacillus subtilis function solely through their external domains in both vegetative and sporulation division.
J Bacteriol: 1999, 181(9);2710-8
[PubMed:10217758] [WorldCat.org] [DOI] (P p)

R A Daniel, E J Harry, V L Katis, R G Wake, J Errington
Characterization of the essential cell division gene ftsL(yIID) of Bacillus subtilis and its role in the assembly of the division apparatus.
Mol Microbiol: 1998, 29(2);593-604
[PubMed:9720875] [WorldCat.org] [DOI] (P p)

V L Katis, E J Harry, R G Wake
The Bacillus subtilis division protein DivIC is a highly abundant membrane-bound protein that localizes to the division site.
Mol Microbiol: 1997, 26(5);1047-55
[PubMed:9426141] [WorldCat.org] [DOI] (P p)

S L Rowland, V L Katis, S R Partridge, R G Wake
DivIB, FtsZ and cell division in Bacillus subtilis.
Mol Microbiol: 1997, 23(2);295-302
[PubMed:9044263] [WorldCat.org] [DOI] (P p)

E J Harry, S L Rowland, M S Malo, R G Wake
Expression of divIB of Bacillus subtilis during vegetative growth.
J Bacteriol: 1994, 176(4);1172-9
[PubMed:8106328] [WorldCat.org] [DOI] (P p)