RibA

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  • Description: GTP cyclohydrolase II/ 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene name ribA
Synonyms
Essential no
Product GTP cyclohydrolase II/ 3,4-dihydroxy-2-butanone 4-phosphate synthase
Function riboflavin biosynthesis
Gene expression levels in SubtiExpress: ribA
Metabolic function and regulation of this protein in SubtiPathways:
Riboflavin / FAD
MW, pI 43 kDa, 5.582
Gene length, protein length 1194 bp, 398 aa
Immediate neighbours ribH, ribE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RibA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RibA expression.png




























Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

FMN-box

The gene

Basic information

  • Locus tag: BSU23260

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2BZ0 (from E. coli, 54% identity, 69% similarity to the C-terminal domain) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism: FMN-box: riboswitch, mediates termination/ antitermination control of the operon, in the absence of FMN: antitermination, in the presence of FMN: termination PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References