GidA

• Description: glucose-inhibited division protein, tRNA uridine 5-carboxymethylaminomethyl modification enzyme
  
  

Gene name	gidA
Synonyms	mnmG
Essential	no
Product	tRNA uridine 5-carboxymethylaminomethyl modification enzyme
Function	tRNA modification
Gene expression levels in SubtiExpress: gidA
Interactions involving this protein in SubtInteract: GidA
MW, pI	69 kDa, 6.326
Gene length, protein length	1884 bp, 628 aa
Immediate neighbours	rsmG, thdF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU41010

Database entries

• BsubCyc: BSU41010

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: mnmG family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GidA-ThdF (suggested by interaction of E. coli counterparts PubMed and by co-localization on the chromosome)

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU41010

• Structure: 3G05 (the protein of E. coli, 51% identity)

• UniProt: P25812

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: thdF-gidA-rsmG-noc PubMed

• Expression browser: gidA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 248 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 813 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 86 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 9 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 14 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: