GlyS

• Description: glycyl-tRNA synthetase (beta subunit)
  
  

Gene name	glyS
Synonyms	yqfK
Essential	yes PubMed
Product	glycyl-tRNA synthetase (beta subunit)
Function	translation
Gene expression levels in SubtiExpress: glyS
Interactions involving this protein in SubtInteract: GlyS
Metabolic function and regulation of this protein in SubtiPathways: glyS
MW, pI	76 kDa, 4.973
Gene length, protein length	2037 bp, 679 aa
Immediate neighbours	ccpN, glyQ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU25260

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU25260

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly) (according to Swiss-Prot)

• Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: GlyQ-GlyS

• Localization:

Database entries

• BsubCyc: BSU25260

• Structure: 1J5W (from Thermotoga maritima, 55% identity, 72% similarity)

• UniProt: P54381

• KEGG entry: [3]

• E.C. number: 6.1.1.14

Expression and regulation

• Operon: glyQ-glyS (according to DBTBS)

• Expression browser: glyS PubMed

• Sigma factor:

• Regulation:
  • induced by glycine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 693 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2795 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1055 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 922 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 982 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Audrey R Nelson, Tina M Henkin, Paul F Agris
tRNA regulation of gene expression: interactions of an mRNA 5'-UTR with a regulatory tRNA.
RNA: 2006, 12(7);1254-61
[PubMed:16741230] [WorldCat.org] [DOI] (P p)

Mary R Yousef, Frank J Grundy, Tina M Henkin
Structural transitions induced by the interaction between tRNA(Gly) and the Bacillus subtilis glyQS T box leader RNA.
J Mol Biol: 2005, 349(2);273-87
[PubMed:15890195] [WorldCat.org] [DOI] (P p)

Frank J Grundy, Mary R Yousef, Tina M Henkin
Monitoring uncharged tRNA during transcription of the Bacillus subtilis glyQS gene.
J Mol Biol: 2005, 346(1);73-81
[PubMed:15663928] [WorldCat.org] [DOI] (P p)

Frank J Grundy, Tina M Henkin
Kinetic analysis of tRNA-directed transcription antitermination of the Bacillus subtilis glyQS gene in vitro.
J Bacteriol: 2004, 186(16);5392-9
[PubMed:15292140] [WorldCat.org] [DOI] (P p)