CheV

• Description: modulation of CheA activity in response to attractants
  
  

Gene name	cheV
Synonyms
Essential	no
Product	CheA modulator
Function	control of CheA activity
Gene expression levels in SubtiExpress: cheV
Interactions involving this protein in SubtInteract: CheV
MW, pI	34 kDa, 4.617
Gene length, protein length	909 bp, 303 aa
Immediate neighbours	ykzT, kre
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription factors and their control, phosphoproteins, motility and chemotaxis

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

• Locus tag: BSU14010

Phenotypes of a mutant

• cheV cheW double mutants exhibit complete loss of chemotaxis PubMed
• not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed

Database entries

• BsubCyc: BSU14010

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): CheW (N-terminal domain of CheV)

Extended information on the protein

• Kinetic information:

• Domains: N-terminal CheW-like domain, C-terminal two-component receiver domain PubMed

• Modification: the C-terminal two-component receiver domain is phosphorylated on a Asp residue by CheA PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • CheA-CheY PubMed

• Localization:
  • forms lateral clusters (phosphorylated form), but in the presence of high asparagine concentration (non-phosphorylated form) there is a reversible re-localization to the poles of the cell PubMed

Database entries

• BsubCyc: BSU14010

• Structure:

• UniProt: P37599

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cheV PubMed

• Expression browser: cheV PubMed

• Sigma factor: SigD PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • in minimal medium, CheV is present with 7,500 +/- 2,000 molecules per cell PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1117 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1764 PubMed

Biological materials

• Mutant:
  • DS70 (cheV::mls in NCIB3610) PubMed
  • TB183 amyE::Phy-sfgfp (cheV::mls in NCIB3610 with constitutive expressed sfgfp) PubMed
  • TB199 amyE::Phy-mKATE2 (cheV::mls in NCIB3610 with constitutive expressed mKATE2) PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: