ZnuA

• Description: ABC transporter for zinc (binding protein)
  
  

Gene name	ycdH
Synonyms
Essential	no
Product	ABC transporter for zinc (binding protein)
Function	zinc uptake
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	35 kDa, 5.146
Gene length, protein length	957 bp, 319 aa
Immediate neighbours	ycdG, ycdI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU02850

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

ABC transporters, trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), membrane proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: bacterial solute-binding protein 9 family (according to Swiss-Prot)

• Paralogous protein(s): MntA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: YcdI-YceA-YcdH PubMed

• Localization:
  • associated to the membrane (via YceA) PubMed
  • extracellular (signal peptide) PubMed

Database entries

• Structure: 2O1E

• UniProt: O34966

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ycdH-ycdI-yceA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by zinc starvation (Zur) PubMed

• Regulatory mechanism:
  • Zur: transcription repression in the presence of zinc PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Ahmed Gaballa, Tao Wang, Rick W Ye, John D Helmann
Functional analysis of the Bacillus subtilis Zur regulon.
J Bacteriol: 2002, 184(23);6508-14
[PubMed:12426338] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

A Gaballa, J D Helmann
Identification of a zinc-specific metalloregulatory protein, Zur, controlling zinc transport operons in Bacillus subtilis.
J Bacteriol: 1998, 180(22);5815-21
[PubMed:9811636] [WorldCat.org] [DOI] (P p)