PbpB

• Description: penicillin-binding protein 2B
  
  

Gene name	pbpB
Synonyms
Essential	yes PubMed
Product	penicillin-binding protein 2B
Function	septation
MW, pI	79 kDa, 9.234
Gene length, protein length	2148 bp, 716 aa
Immediate neighbours	ftsL, spoVD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell wall synthesis, essential genes, membrane proteins

The gene

Basic information

• Locus tag: BSU15160

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: transpeptidase family (according to Swiss-Prot)

• Paralogous protein(s): SpoVD

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • folding requires PrsA PubMed
  • PbpB-DivIB-(FtsL-DivIC)2 PubMed, as part of the divisome
  • WalK-PbpB PubMed

• Localization: cell membrane PubMed, extracellular (signal peptide) PubMed, localizes to the sporulation septum during sporulation PubMed

Database entries

• Structure:

• UniProt: Q07868

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mraZ-mraW-ftsL-pbpB PubMed

• Sigma factor:

• Regulation:
  • constitutively expressed PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jeff Errington lab

• Antibody:

Labs working on this gene/protein

Jeff Errington, Newcastle University, UK homepage