Scr

• Description: 4.5 S RNA
  
  

Gene name	scr
Synonyms
Essential	no
Product	small cytoplasmic RNA (scRNA, 4.5S RNA), signal recognition particle-like (SRP) component
Function	presecretory protein translocation
Interactions involving this RNA in SubtInteract: Scr
Immediate neighbours	yaaJ, dnaX
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

protein secretion, ncRNA

The gene

Basic information

• Locus tag:

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

• Structure:

The RNA

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

Extended information on the RNA

• Kinetic information

• Interactions:
  • Scr-Ffh in the signal recognition particle

• Localization

Database entries

• Structure:

Expression and regulation

• Operon: yaaJ-scr, scr

• Sigma factor: SigA (both promoters)

• Regulation:

• Regulatory mechanism:

• Additional information: transcribed as 354 nt precursor, cleaved at the 5' end by RNase III and at the 3' site by RNase J1 to yield a 275 nt molecule, final trimming to 271 nt sc RNA by RNase PH PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

References

Shiyi Yao, Joshua B Blaustein, David H Bechhofer
Processing of Bacillus subtilis small cytoplasmic RNA: evidence for an additional endonuclease cleavage site.
Nucleic Acids Res: 2007, 35(13);4464-73
[PubMed:17576666] [WorldCat.org] [DOI] (I p)

H Takamatsu, K Bunai, T Horinaka, A Oguro, K Nakamura, K Watabe, K Yamane
Identification of a region required for binding to presecretory protein in Bacillus subtilis Ffh, a homologue of the 54-kDa subunit of mammalian signal recognition particle.
Eur J Biochem: 1997, 248(2);575-82
[PubMed:9346318] [WorldCat.org] [DOI] (P p)

J C Struck, R K Hartmann, H Y Toschka, V A Erdmann
Transcription and processing of Bacillus subtilis small cytoplasmic RNA.
Mol Gen Genet: 1989, 215(3);478-82
[PubMed:2468993] [WorldCat.org] [DOI] (P p)