FabF

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  • Description: beta-ketoacyl-acyl carrier protein synthase II, involved in the control of membrane fluidity

Gene name fabF
Synonyms yjaY
Essential yes PubMed
Product beta-ketoacyl-acyl carrier protein synthase II
Function fatty acid biosynthesis
Metabolic function and regulation of this protein in SubtiPathways:
Lipid synthesis
MW, pI 43 kDa, 4.768
Gene length, protein length 1239 bp, 413 aa
Immediate neighbours fabHA, yjaZ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
FabF context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

membrane dynamics, biosynthesis of lipids, cell envelope stress proteins (controlled by SigM, V, W, X, Y), essential genes

This gene is a member of the following regulons

FapR regulon, SigW regulon

The gene

Basic information

  • Locus tag: BSU11340

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • (Z)-hexadec-11-enoyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = (Z)-3-oxooctadec-13-enoyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein] (according to Swiss-Prot)
    • SigW-dependent expression of fabF and the yuaF-floT-yuaI operon result in reduced membrane fluidity PubMed
  • Protein family: beta-ketoacyl-ACP synthases family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1OX0 (the protein from Streptococcus pneumoniae) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • fabHA: expressed when the cells experience a lack of malonyl-CoA (FapR) PubMed
    • fabHA: induced upon fatty acid biosynthesis inhibition PubMed
    • fabF: induced by alkaline shock and by polymyxin B, vancomycin, cephalosporin C, D-cycloserine, and triton X-100 (SigW) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Stephen W White, Jie Zheng, Yong-Mei Zhang, Rock
The structural biology of type II fatty acid biosynthesis.
Annu Rev Biochem: 2005, 74;791-831
[PubMed:15952903] [WorldCat.org] [DOI] (P p)

Original Publications

Additional publications: PubMed