CwlO

• Description: D,L-endopeptidase-type autolysin
  
  

Gene name	cwlO
Synonyms	yzkA, yvcE
Essential	no
Product	endopeptidase-type autolysin
Function	cell wall synthesis, cell proliferation
Gene expression levels in SubtiExpress: cwlO
MW, pI	50 kDa, 5.326
Gene length, protein length	1419 bp, 473 aa
Immediate neighbours	trxB, yvcD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover

This gene is a member of the following regulons

WalR regulon

The gene

Basic information

• Locus tag: BSU34800

Phenotypes of a mutant

a cwlO lytE mutant is not viable PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: peptidase C40 family (according to Swiss-Prot)

• Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity

Extended information on the protein

• Kinetic information:

• Domains:
  • C-terminal D,L-endopeptidase domain PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (according to Swiss-Prot)
  • extracellular (signal peptide) PubMed
  • localizes to the lateral sidewall of the cell (via the N-terminal domain) PubMed

Database entries

• Structure:

• UniProt: P40767

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: cwlO PubMed

• Sigma factor: SigA (according to PubMed)

• Regulation: activated by WalR PubMed

• Regulatory mechanism:

• Additional information:
  • The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Waldemar Vollmer
Bacterial growth does require peptidoglycan hydrolases.
Mol Microbiol: 2012, 86(5);1031-5
[PubMed:23066944] [WorldCat.org] [DOI] (I p)

Original publications

Additional publications: PubMed