KinA

• Description: two-component sensor kinase, phosphorylates Spo0F, part of the phosphorelay
  
  

Gene name	kinA
Synonyms	spoIIF, spoIIJ, gsiC, scoB, scoD
Essential	no
Product	two-component sensor kinase
Function	initiation of sporulation
Gene expression levels in SubtiExpress: kinA
Interactions involving this protein in SubtInteract: KinA
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	68 kDa, 5.491
Gene length, protein length	1818 bp, 606 aa
Immediate neighbours	pbpH, patA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, transcription factors and their control, phosphorelay, phosphoproteins

This gene is a member of the following regulons

SigH regulon, Spo0A regulon, stringent response

The gene

Basic information

• Locus tag: BSU13990

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • autophosphorylation, phosphorylation of Spo0F
  • mainly active in the older, inner regions of a colony (with KinB) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • three tandem PAS domains in the N-terminal region of KinA, the second PAS domain is the major N-terminal determinant of KinA dimerization PubMed
  • the first PAS domain is required for NAD(+) binding PubMed
  • C-terminal histidine phosphotransferase domain

• Modification: autophosphorylation on a His residue

• Cofactor(s):

• Effectors of protein activity:
  • interaction with KipI or Sda inhibits autophosphorylation of KinA PubMed
  • autophosphorylation is inhibited by SivA and BslA PubMed
  • activated by interaction with NAD(+) that indicated poor respiratory activity of the cell PubMed

• Interactions:
  • forms tetramers PubMed
  • KinA-KipI PubMed
  • KinA-Sda PubMed
  • Spo0F-KinA

• Localization:
  • cytoplasm

Database entries

• Structure: 2VLG (PAS domain)

• UniProt: P16497

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: kinA PubMed

• Expression browser: kinA PubMed

• Sigma factor: SigH PubMed

• Regulation:
  • expressed under conditions that trigger sporulation (Spo0A) PubMed
  • induced upon addition of decoyinine (positive stringent response) PubMed

• Regulatory mechanism:
  • Spo0A: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Liza Gross
Built-in timer delays differentiation.
PLoS Biol: 2012, 10(1);e1001254
[PubMed:22303284] [WorldCat.org] [DOI] (I p)