HutH

• Description: histidase
  
  

Gene name	hutH
Synonyms
Essential	no
Product	histidase
Function	histidine utilization
Gene expression levels in SubtiExpress: hutH
Metabolic function and regulation of this protein in SubtiPathways: His
MW, pI	55 kDa, 5.221
Gene length, protein length	1524 bp, 508 aa
Immediate neighbours	hutP, hutU
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

CcpA regulon, CodY regulon, HutP regulon

The gene

Basic information

• Locus tag: BSU39350

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-histidine = urocanate + NH₃ (according to Swiss-Prot)

• Protein family: PAL/histidase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1B8F (from Pseudomonas putida, 43% identity, 62% similarity) PubMed

• UniProt: P10944

• KEGG entry: [3]

• E.C. number: 4.3.1.3

Expression and regulation

• Operon: hutP-hutH-hutU-hutI-hutG-hutM PubMed

• Expression browser: hutH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by histidine (HutP) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • CodY: transcription repression PubMed
  • HutP: transcriptional antitermination at a protein-dependent RNA switch located between hutP and hutH PubMed

• Additional information:

Biological materials

• Mutant: GP1114 (spc), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Robert A Bender
Regulation of the histidine utilization (hut) system in bacteria.
Microbiol Mol Biol Rev: 2012, 76(3);565-84
[PubMed:22933560] [WorldCat.org] [DOI] (I p)