Pta

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  • Description: phosphotransacetylase

Gene name pta
Synonyms ipa-88d, ywfJ
Essential no
Product phosphotransacetylase
Function overflow metabolism
Gene expression levels in SubtiExpress: pta
Metabolic function and regulation of this protein in SubtiPathways:
pta
MW, pI 34 kDa, 4.649
Gene length, protein length 969 bp, 323 aa
Immediate neighbours cysL, ywfI
Sequences Protein DNA DNA_with_flanks
Genetic context
Pta context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Pta expression.png
















Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

  • Locus tag: BSU37660

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Acetyl-CoA + phosphate = CoA + acetyl phosphate (according to Swiss-Prot)
  • Protein family: phosphate acetyltransferase and butyryltransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on (Ser-123 OR Thr-128 OR Ser-129) PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1XCO (complex with acetylphosphate), 1TD9
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expression activated by glucose (2.6 fold) (CcpA) PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 2825 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 7432 PubMed

Biological materials

  • Mutant: GP668 (aphA3), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Alan J Wolfe
The acetate switch.
Microbiol Mol Biol Rev: 2005, 69(1);12-50
[PubMed:15755952] [WorldCat.org] [DOI] (P p)

Original publications

Additional publications: PubMed