RecO

• Description: mediator of RecA binding to ssDNA, required for the formation of RecA DNA repair centers
  
  

Gene name	recO
Synonyms	yqxN, yqfI
Essential	no
Product	mediator of RecA binding to ssDNA
Function	DNA repair/ recombination
Gene expression levels in SubtiExpress: recO
Interactions involving this protein in SubtInteract: RecO
MW, pI	29 kDa, 8.208
Gene length, protein length	765 bp, 255 aa
Immediate neighbours	glyQ, yqzL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

The gene

Basic information

• Locus tag: BSU25280

Phenotypes of a mutant

• drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) PubMed

Database entries

• BsubCyc: BSU25280

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • provides RecA access to ssDNA during chromosomal transformation (together with DprA) PubMed
  • catalyzes annealing of SsbA or SsbA/SsbB coated ssDNAs to allow the formation of DNA duplexes with tails during plasmid transformation PubMed
  • required for the formation of RecA DNA repair centers (together with RecR) PubMed

• Protein family: recO family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SsbA (bound to ssDNA) -RecO, this helps to dislodge SsbA and SsbB from ssDNA PubMed
  • RecR-RecO, RecR:RecO molecular ratio of 2:1 (as judged from other bacteria such as E. coli and Deinococcus radiodurans) PubMed

• Localization:
  • cytoplasm (according to Swiss-Prot)
  • localizes to the DNA entry pole during transformation PubMed

Database entries

• BsubCyc: BSU25280

• Structure:
  • 2V1C (the RecR-RecO complex from Deinococcus radiodurans, RecR: 52% identity, 78% similarity, RecO: 30%/ 58%) PubMed

• UniProt: P42095

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: recO PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A892 ( recO::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: