BglH

• Description: phospho-beta-glucosidase
  
  

Gene name	bglH
Synonyms
Essential	no
Product	phospho-beta-glucosidase
Function	salicin utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	53 kDa, 4.957
Gene length, protein length	1407 bp, 469 aa
Immediate neighbours	yxiE, bglP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU39260

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H₂O = D-glucose + D-glucose 6-phosphate (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 1 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: P40740

• KEGG entry: [3]

• E.C. number: 3.2.1.86

Expression and regulation

• Operon: bglP-bglH-yxiE PubMed

• Sigma factor: SigA

• Regulation: repressed by glucose (CcpA) , induced by salicin PubMed, repressed by glucose (catabolite repression)

• Regulatory mechanism: CcpA: transcription repression, Induction: LicT-dependent RNA switch (antitermination), catabolite repression: repression by CcpA (CcpA binding site overlaps -35 region) and lack of LicT-dependent antitermination in the presence of gucose due to the requirement of LicT to be phosphorylated by HPr

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Le Coq, D., Lindner, C., Krüger, S., Steinmetz, M. & Stülke, J. (1995) New ß-glucosides (bgl) genes in Bacillus subtilis: The bglP gene product has both transport and regulatory functions, similar to that of the Escherichia coli BglF protein. J. Bacteriol. 177: 1527-1535. PubMed
2. Krüger, S. and Hecker, M. (1995) Regulation of the putative bglPH operon for aryl-ß-glycoside utilization in Bacillus subtilis. J. Bacteriol. 177, 5590-5597. PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed