Bcd

• Description: valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenase
  
  

Gene name	bcd
Synonyms	bkd, yqiT
Essential	no
Product	valine dehydrogenase, isoleucine dehydrogenase, L-leucine dehydrogenase
Function	utilization of branched-chain keto acids
Metabolic function and regulation of this protein in SubtiPathways: Ile, Leu, Val
MW, pI	39 kDa, 4.942
Gene length, protein length	1092 bp, 364 aa
Immediate neighbours	buk, ptb
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU24080

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-leucine + H₂O + NAD⁺ = 4-methyl-2-oxopentanoate + NH₃ + NADH (according to Swiss-Prot)

• Protein family: Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P54531

• KEGG entry: [3]

• E.C. number: 1.4.1.9

Expression and regulation

• Operon: ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB

• Sigma factor: SigL PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: