AcoA

• Description: acetoin dehydrogenase E1 component (TPP-dependent alpha subunit)
  
  

Gene name	acoA
Synonyms	yfjK
Essential	no
Product	acetoin dehydrogenase E1 component (TPP-dependent alpha subunit)
Function	acetoin utilization
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	35 kDa, 4.912
Gene length, protein length	999 bp, 333 aa
Immediate neighbours	yfjL, acoB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU08060

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: (AcoA-AcoB)-AcoC-AcoL

• Localization: cytoplasm (homogeneous) PubMed

Database entries

• Structure:

• UniProt: O31404

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: acoA-acoB-acoC-acoL PubMed

• Sigma factor: SigL PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by acetoin (AcoR) PubMed
  • induced under anaerobic conditions (Fnr) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • AcoR: transcription activation (interaction with SigL-containing RNA polymerase) PubMed
  • Fnr: indirect positive regulation via NarG-NarH-NarJ-NarI PubMed

• Additional information:the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage