GltB

• Description: small subunit of glutamate synthase
  
  

Gene name	gltB
Synonyms
Essential	no
Product	glutamate synthase (small subunit)
Function	glutamate biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate
MW, pI	54.6 kDa, 7.69
Gene length, protein length	1479 bp, 493 amino acids
Immediate neighbours	yogA, gltA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU18440

Phenotypes of a mutant

auxotrophic for glutamate

Database entries

• DBTBS entry: [1]

• SubtiList entry:[2]

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2 L-glutamate + NADP⁺ = L-glutamine + 2-oxoglutarate + NADPH (according to Swiss-Prot) 2 L-glutamate + NADP(+) <=> L-glutamine + 2-oxoglutarate + NADPH

• Protein family: glutamate synthase family.

• Paralogous protein(s): none

Extended information on the protein

• Kinetic information:

• Domains:
  • nucleotide binding domain (NADP) (299–313)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: GltA-GltB PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O34399

• KEGG entry: [3]

• E.C. number: 1.4.1.13

Expression and regulation

• Operon: gltA-gltB PubMed

• Sigma factor: SigA PubMed

• Regulation: see gltA

• Additional information:

Biological materials

• Mutant: GP807 (del gltAB::tet), GP517 (ermC), both available in Stülke lab

• Expression vector:
  • pGP1119 (in pGP380, for SPINE, expression in B. subtilis), available in Stülke lab

• lacZ fusion: see gltA

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• Antibody:

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

Jörg Stülke, University of Göttingen, Germany Homepage