NfrA

• Description: Spx-dependent FMN-containing NADPH-linked nitro/flavin reductase, stress protein
  
  

Gene name	nfrA
Synonyms	ywcG, ipa-43d
Essential	no
Product	FMN-containing NADPH-linked nitro/flavin reductase
Function	unknown
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	28 kDa, 5.732
Gene length, protein length	747 bp, 249 aa
Immediate neighbours	ywcH, rodA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

electron transport/ other, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

SigD regulon, Spo0A regulon, Spx regulon

The gene

Basic information

• Locus tag: BSU38110

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: possesses an NADH oxidase activity that leads to high concentrations of oxygen peroxide and an NAD(+) degrading activity leading to free nicotinamide PubMed

• Protein family: flavin oxidoreductase frp family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3N2S PubMed

• UniProt: P39605

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nfrA-ywcH PubMed

• Sigma factor: SigA PubMed, SigD PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • Spx: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: