PdaC

• Description: peptidoglycan deacetylase C
  
  

Gene name	yjeA
Synonyms
Essential	no
Product	polysaccharide deacetylase C
Function	cell wall modification
MW, pI	53 kDa, 9.105
Gene length, protein length	1401 bp, 467 aa
Immediate neighbours	cotT, yjfA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell wall/ other

This gene is a member of the following regulons

WalR regulon

The gene

Basic information

• Locus tag: BSU12100

Phenotypes of a mutant

• sensitive to lysozyme treatment PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • deacetylates N-acetylmuramic acid (MurNAc) but not GlcNAc from peptidoglycan PubMed

• Protein family: polysaccharide deacetylase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:
  • K(m)=4.8 mM and k(cat)=0.32 s(-1) (toward B. subtilis peptidoglycan) PubMed
• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O34798

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation: repessed by WalR PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kaori Kobayashi, I Putu Sudiarta, Takeko Kodama, Tatsuya Fukushima, Katsutoshi Ara, Katsuya Ozaki, Junichi Sekiguchi
Identification and characterization of a novel polysaccharide deacetylase C (PdaC) from Bacillus subtilis.
J Biol Chem: 2012, 287(13);9765-9776
[PubMed:22277649] [WorldCat.org] [DOI] (I p)

Ka-Lun Ng, Chui-Chi Lam, Zhibiao Fu, Yi-Fan Han, Karl W K Tsim, Wan-Keung R Wong
Cloning and characterization of the yjeA gene, encoding a novel deoxyribonuclease, from Bacillus subtilis.
J Biochem: 2007, 142(5);647-54
[PubMed:17878218] [WorldCat.org] [DOI] (P p)

Paola Bisicchia, David Noone, Efthimia Lioliou, Alistair Howell, Sarah Quigley, Thomas Jensen, Hanne Jarmer, Kevin M Devine
The essential YycFG two-component system controls cell wall metabolism in Bacillus subtilis.
Mol Microbiol: 2007, 65(1);180-200
[PubMed:17581128] [WorldCat.org] [DOI] (P p)