RapH

• Description: response regulator aspartate phosphatase, dephosphorylates Spo0F-P, control of the phosphorelay, control of ComA activity
  
  

Gene name	rapH
Synonyms	yeeH, yzqA
Essential	no
Product	response regulator aspartate phosphatase
Function	control of sporulation initiation and ComA activity
Interactions involving this protein in SubtInteract: RapH
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	49 kDa, 6.121
Gene length, protein length	1293 bp, 431 aa
Immediate neighbours	yeeG, phrH
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

protein modification, transcription factors and their control, phosphorelay, quorum sensing

This gene is a member of the following regulons

AbrB regulon, ComK regulon, RghR regulon

The gene

Basic information

• Locus tag: BSU06830

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • binds to and thereby inhibits ComA activity PubMed
  • RapH dephosphorylates Spo0F-P and thereby controls the phosphorelay PubMed
  • in addition to dephosphorylating Spo0F, RapH can antagonize sporulation by sterically blocking phosphoryl transfer to and from Spo0F PubMed

• Protein family: RAP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • interaction with PhrH inhibits dephosphorylation of Spo0F and sequestration of ComA by RapH PubMed

• Interactions:
  • RapH-ComA PubMed
  • RapH-Spo0F-P PubMed
  • RapH-PhrH PubMed

• Localization:

Database entries

• Structure: 3Q15 (RapH-Spo0F complex) PubMed

• UniProt: P40771

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rapH-phrH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by RghR PubMed

• Regulatory mechanism:
  • RghR: transcription repression PubMed
  • ComK: transcription activation PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed