EpsB

• Description: extracellular polysaccharide synthesis, putative protein tyrosine kinase
  
  

Gene name	epsB
Synonyms	yveL
Essential	no
Product	unknown
Function	biofilm formation
Gene expression levels in SubtiExpress: epsB
Interactions involving this protein in SubtInteract: EpsB
Regulation of this protein in SubtiPathways: Biofilm
MW, pI	24 kDa, 9.918
Gene length, protein length	681 bp, 227 aa
Immediate neighbours	epsC, epsA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, biofilm formation

This gene is a member of the following regulons

AbrB regulon, SinR regulon

The gene

Basic information

• Locus tag: BSU34360

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate (according to Swiss-Prot)

• Protein family: BY kinase, see the Bacterial Protein Tyrosine Kinase Database)

• Paralogous protein(s): PtkA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • EpsA-EpsB (based on TkmA-PtkA interaction)

• Localization:

Database entries

• Structure: 2VED (CapB, the homolog in Staphylococcus aureus) PubMed

• UniProt: P71051

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO PubMed

• Expression browser: epsB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by SinR PubMed

• Regulatory mechanism:
  • SinR: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:
  • induction by sequestration of SinR by SinI or SlrA PubMed
  • the epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO operon is not expressed in a ymdB mutant PubMed
  • the amount of the mRNA is substantially decreased upon depletion of RNase Y (this is likely due to the increased stability of the sinR mRNA) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

• FLAG-tag construct: GP1541 (epsB-3xFLAG spc trpC2) , available in Jörg Stülke's lab

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage