RnhB

• Description: RNase HII, endoribonuclease
  
  

Gene name	rnhB
Synonyms	rnh
Essential	no
Product	Mn2+-dependent RNase HII
Function	endonucleolytic cleavage of RNA in RNA-DNA hybrid molecules
Gene expression levels in SubtiExpress: rnhB
MW, pI	28 kDa, 5.518
Gene length, protein length	765 bp, 255 aa
Immediate neighbours	rbgA, ylqG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, Rnases

The gene

Basic information

• Locus tag: BSU16060

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Endonucleolytic cleavage to 5'-phosphomonoester (according to Swiss-Prot)

• Protein family: RNase HII family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O31744

• KEGG entry: [2]

• E.C. number: 3.1.26.4.

Expression and regulation

• Operon:

• Expression browser: rnhB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Takashi Tadokoro, Shigenori Kanaya
Ribonuclease H: molecular diversities, substrate binding domains, and catalytic mechanism of the prokaryotic enzymes.
FEBS J: 2009, 276(6);1482-93
[PubMed:19228197] [WorldCat.org] [DOI] (I p)