CtaC

• Description: cytochrome-c oxidase (subunit II)
  
  

Gene name	ctaC
Synonyms
Essential	no
Product	cytochrome-c oxidase (subunit II)
Function	respiration
Gene expression levels in SubtiExpress: ctaC
Interactions involving this protein in SubtInteract: CtaC
MW, pI	39 kDa, 8.635
Gene length, protein length	1068 bp, 356 aa
Immediate neighbours	ctaB, ctaD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

respiration, membrane proteins

This gene is a member of the following regulons

Abh regulon, AbrB regulon, CcpA regulon, ResD regulon

The gene

Basic information

• Locus tag: BSU14890

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 4 ferrocytochrome c + O₂ + 4 H⁺ = 4 ferricytochrome c + 2 H₂O (according to Swiss-Prot)

• Protein family: cytochrome c oxidase subunit 2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): 2 heme A molecules, Cu(A) PubMed

• Effectors of protein activity:

• Interactions:
  • Sco-CtaC PubMed
  • KinB-CtaC PubMed
  • part of a respiratory supercomplex PubMed
  • CtaC-CtaE PubMed

• Localization:
  • cell membrane PubMed

Database entries

• Structure:

• UniProt: P24011

• KEGG entry: [3]

• E.C. number: 1.9.3.1

Expression and regulation

• • Operon: ctaB-ctaC-ctaD-ctaE-ctaF-ctaG PubMed

• Expression browser: ctaC PubMed

• Sigma factor:

• Regulation:
  • expressed under anaerobic conditions (ResD) PubMed
  • repressed by glucose (11-fold) (CcpA) PubMed

• Regulatory mechanism:
  • ResD: transcription activation PubMed
  • CcpA: transcription repression
  • Abh and AbrB: synergistic transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Pedro M F Sousa, Marco A M Videira, Filipe A S Santos, Brian L Hood, Thomas P Conrads, Ana M P Melo
The bc:caa3 supercomplexes from the Gram positive bacterium Bacillus subtilis respiratory chain: a megacomplex organization?
Arch Biochem Biophys: 2013, 537(1);153-60
[PubMed:23880299] [WorldCat.org] [DOI] (I p)

Ilana Kolodkin-Gal, Alexander K W Elsholz, Christine Muth, Peter R Girguis, Roberto Kolter, Richard Losick
Respiration control of multicellularity in Bacillus subtilis by a complex of the cytochrome chain with a membrane-embedded histidine kinase.
Genes Dev: 2013, 27(8);887-99
[PubMed:23599347] [WorldCat.org] [DOI] (I p)

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

N R Mattatall, J Jazairi, B C Hill
Characterization of YpmQ, an accessory protein required for the expression of cytochrome c oxidase in Bacillus subtilis.
J Biol Chem: 2000, 275(37);28802-9
[PubMed:10837475] [WorldCat.org] [DOI] (P p)

N Azarkina, S Siletsky, V Borisov, C von Wachenfeldt, L Hederstedt, A A Konstantinov
A cytochrome bb'-type quinol oxidase in Bacillus subtilis strain 168.
J Biol Chem: 1999, 274(46);32810-7
[PubMed:10551842] [WorldCat.org] [DOI] (P p)

J Bengtsson, H Tjalsma, C Rivolta, L Hederstedt
Subunit II of Bacillus subtilis cytochrome c oxidase is a lipoprotein.
J Bacteriol: 1999, 181(2);685-8
[PubMed:9882689] [WorldCat.org] [DOI] (P p)

X Liu, H W Taber
Catabolite regulation of the Bacillus subtilis ctaBCDEF gene cluster.
J Bacteriol: 1998, 180(23);6154-63
[PubMed:9829923] [WorldCat.org] [DOI] (P p)

J van der Oost, C von Wachenfeld, L Hederstedt, M Saraste
Bacillus subtilis cytochrome oxidase mutants: biochemical analysis and genetic evidence for two aa3-type oxidases.
Mol Microbiol: 1991, 5(8);2063-72
[PubMed:1685007] [WorldCat.org] [DOI] (P p)