RnpB

• Description: RNA component of RNase P
  
  

Gene name	rnpB
Synonyms	rnaP
Essential	yes PubMed
Product	RNA component of ribonuclease P (RNase P) (catalytic subunit, ribozyme)
Function	cleavage of precursor sequences from the 5' ends of pre-tRNAs
Interactions involving this RNA in SubtInteract: RnpB
Gene length	401 bp
Immediate neighbours	ypsC, ypsB
Gene sequence (+200bp)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

Rnases, translation, ncRNA, essential genes

The gene

Basic information

• Locus tag:

Phenotypes of a mutant

• essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

• Structure:
  • 2A64 (from Geobacillus stearothermophilus)
  • 1NBS (specificity domain)
  • 3OKB (complex of RnpA with the RNA RnpB and tRNA, from Thermotoga maritima) PubMed

The RNA

• Biological activity: 5' end maturation of precursor tRNAs

• RNA family:

• cofactor: divalent cations (Ca(II) or Mg (II)) for the stabilization of the complex with pre-tRNA PubMed

• Interactions: RnpA-RnpB PubMed

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/RNA

• Carol Fierke, University of Michigan, Ann Arbor, USA homepage
• Roland Hartmann, Marburg University, Germany homepage

References

Reviews

Roland K Hartmann, Markus Gössringer, Bettina Späth, Susan Fischer, Anita Marchfelder
The making of tRNAs and more - RNase P and tRNase Z.
Prog Mol Biol Transl Sci: 2009, 85;319-68
[PubMed:19215776] [WorldCat.org] [DOI] (P p)

J Kristin Smith, John Hsieh, Carol A Fierke
Importance of RNA-protein interactions in bacterial ribonuclease P structure and catalysis.
Biopolymers: 2007, 87(5-6);329-38
[PubMed:17868095] [WorldCat.org] [DOI] (P p)

Alexei V Kazantsev, Norman R Pace
Bacterial RNase P: a new view of an ancient enzyme.
Nat Rev Microbiol: 2006, 4(10);729-40
[PubMed:16980936] [WorldCat.org] [DOI] (I p)

Donald Evans, Steven M Marquez, Norman R Pace
RNase P: interface of the RNA and protein worlds.
Trends Biochem Sci: 2006, 31(6);333-41
[PubMed:16679018] [WorldCat.org] [DOI] (P p)

Alfredo Torres-Larios, Kerren K Swinger, Tao Pan, Alfonso Mondragón
Structure of ribonuclease P--a universal ribozyme.
Curr Opin Struct Biol: 2006, 16(3);327-35
[PubMed:16650980] [WorldCat.org] [DOI] (P p)

John Hsieh, Andy J Andrews, Carol A Fierke
Roles of protein subunits in RNA-protein complexes: lessons from ribonuclease P.
Biopolymers: 2004, 73(1);79-89
[PubMed:14691942] [WorldCat.org] [DOI] (P p)

Enno Hartmann, Roland K Hartmann
The enigma of ribonuclease P evolution.
Trends Genet: 2003, 19(10);561-9
[PubMed:14550630] [WorldCat.org] [DOI] (P p)

Original Publications

Additional publications: PubMed