NasE

• Description: assimilatory nitrite reductase (subunit)
  
  

Gene name	nasE
Synonyms	nasBD, nirD
Essential	no
Product	assimilatory nitrite reductase (subunit)
Function	utilization of nitrite as nitrogen source
Gene expression levels in SubtiExpress: nasE
Interactions involving this protein in SubtInteract: NasE
Metabolic function and regulation of this protein in SubtiPathways: nasE
MW, pI	11 kDa, 4.805
Gene length, protein length	318 bp, 106 aa
Immediate neighbours	nasF, nasD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

respiration, utilization of nitrogen sources other than amino acids

This gene is a member of the following regulons

Fur regulon, NsrR regulon, ResD regulon, TnrA regulon

The gene

Basic information

• Locus tag: BSU03290

Database entries

• BsubCyc: BSU03290

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Ammonium hydroxide + 3 NAD(P)⁺ + H₂O = nitrite + 3 NAD(P)H (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:
  • NAD PubMed

• Effectors of protein activity:

• Interactions: NasD-NasE PubMed

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU03290

• Structure:

• UniProt: P42436

• KEGG entry: [3]

• E.C. number: 1.7.1.4

Expression and regulation

• Operon:
  • nasB-nasC-nasD-nasE-nasF PubMed
  • nasD-nasE-nasF PubMed

• Expression browser: nasE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • nasD: expressed under anaerobic conditions (ResD) PubMed
  • induced by nitric oxide under anaerobic conditions (NsrR) PubMed
  • induced by iron starvation (Fur) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • ResD: transcription activation PubMed
  • NsrR: transcription repression PubMed
  • Fur: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: