MurAA

• Description: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
  
  

Gene name	murAA
Synonyms	murA
Essential	yes PubMed
Product	UDP-N-acetylglucosamine 1-carboxyvinyltransferase
Function	peptidoglycan precursor biosynthesis
Gene expression levels in SubtiExpress: murAA
Metabolic function and regulation of this protein in SubtiPathways: murAA
MW, pI	46 kDa, 5.45
Gene length, protein length	1308 bp, 436 aa
Immediate neighbours	spoIID, ywmB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes

The gene

Basic information

• Locus tag: BSU36760

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU36760

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine (according to Swiss-Prot)

• Protein family: MurA subfamily (according to Swiss-Prot)

• Paralogous protein(s): MurAB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU36760

• Structure: 3SG1 (from B. anthracis, 79% identity, 94% similarity)

• UniProt: P70965

• KEGG entry: [3]

• E.C. number: 2.5.1.7

Additional information

• MurAA is subject to ClpC-ClpP-dependent degradation upon glucose starvation PubMed

Expression and regulation

• Operon: murAA PubMed

• Expression browser: murAA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant:

• Expression vector:
  • pRSETA-murAA, Expression of His(6)-MurAA, avalable in Ulf Gerth's lab PubMed
  • pGP2597: (IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172), available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: avalable in Ulf Gerth's lab PubMed

References

Reviews

Ankur Gautam, Praveen Rishi, Rupinder Tewari
UDP-N-acetylglucosamine enolpyruvyl transferase as a potential target for antibacterial chemotherapy: recent developments.
Appl Microbiol Biotechnol: 2011, 92(2);211-25
[PubMed:21822642] [WorldCat.org] [DOI] (I p)