RatA

• Description: regulatory RNA, ncRNA, antisense for txpA
  
  

Gene name	ratA
Synonyms
Essential	no
Product	txpA antisense RNA
Function	control of txpA expression
Gene length	222 bp
Immediate neighbours	txpA, bsrH
Gene sequence (+200bp)
Genetic context File:GenE context.gif
Expression at a glance   PubMed

Categories containing this gene/protein

toxins, antitoxins and immunity against toxins, Skin element, ncRNA

The gene

Basic information

• Locus tag:

Database entries

• BsubCyc: ?
• BsubCyc: ?

• DBTBS entry: no entry

• SubtiList entry:

• Structure:

The RNA

binds the txpA mRNA and triggers its degradation PubMed

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • the txpA mRNA-RatA RNA duplex is degraded by RNase III PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Richard Losick, Harvard University, Cambridge, USA Homepage

References

Reviews

Fabian M Commichau, Jörg Stülke
A mystery unraveled: essentiality of RNase III in Bacillus subtilis is caused by resident prophages.
PLoS Genet: 2012, 8(12);e1003199
[PubMed:23300472] [WorldCat.org] [DOI] (I p)

Original publications

Durand S, Gilet L, Condon C  
The essential function of B. subtilis RNase III is to silence foreign toxin genes. 
PLoS Genet. 2012 8(12): e1003181.
PubMed:23300471

Jessica M Silvaggi, John B Perkins, Richard Losick
Small untranslated RNA antitoxin in Bacillus subtilis.
J Bacteriol: 2005, 187(19);6641-50
[PubMed:16166525] [WorldCat.org] [DOI] (P p)