YtsJ

• Description: malic enzyme, forms a transhydrogenation cycle with MalS for balancing of NADPH
  
  

Gene name	ytsJ
Synonyms
Essential	no
Product	NADP-dependent malate dehydrogenase
Function	malate utilization
Gene expression levels in SubtiExpress: ytsJ
Metabolic function and regulation of this protein in SubtiPathways: ytsJ
MW, pI	43 kDa, 5.046
Gene length, protein length	1230 bp, 410 aa
Immediate neighbours	accD, dnaE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, most abundant proteins

The gene

Basic information

• Locus tag: BSU29220

Phenotypes of a mutant

Poor growth with malate as single carbon source PubMed

Database entries

• BsubCyc: BSU29220

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-malate + NADP⁺ = pyruvate + CO₂ + NADPH (according to Swiss-Prot) malate <--> pyruvate

• Protein family: malic enzymes family (according to Swiss-Prot)

• Paralogous protein(s): MleA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • Cytoplasm (Homogeneous) PubMed

Database entries

• BsubCyc: BSU29220

• Structure: 1WW8 (from Pyrococcus horikoshii, 46% identity, 63% similarity)

• UniProt: O34962

• KEGG entry: [3]

• E.C. number: 1.1.1.38

Expression and regulation

• Operon: ytsJ PubMed

• Expression browser: ytsJ PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 3654 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 4845 PubMed

Biological materials

• Mutant:
  • GP612 (spc), available in Jörg Stülke's lab
  • GP1143 (spc), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion: GP1432 (spc, based on pGP1870), available in the Stülke lab

• two-hybrid system:

• FLAG-tag construct: GP1131 (spc, based on pGP1331), available in Jörg Stülke's lab

• Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France