IseA

• Description: inhibits in vitro activity of cell wall endopeptidases LytE and LytF, inhibits cell separation
  
  

Gene name	iseA
Synonyms	yoeB
Essential	no
Product	inhibitor of autolysins
Function	protection against cell envelope stress
Gene expression levels in SubtiExpress: iseA
Interactions involving this protein in SubtInteract: IseA
MW, pI	19 kDa, 10.176
Gene length, protein length	543 bp, 181 aa
Immediate neighbours	yoeA, trnSL-Arg1
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover, membrane proteins

This gene is a member of the following regulons

WalR regulon

The gene

Basic information

• Locus tag: BSU18380

Database entries

• BsubCyc: BSU18380

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • IseA-LytF (catalytic domain) PubMed

• Localization: cell membrane (according to Swiss-Prot), localized to cell separation sites

Database entries

• BsubCyc: BSU18380

• Structure:

• UniProt: O34841

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: iseA PubMed

• Expression browser: iseA PubMed

• Sigma factor:

• Regulation:
  • repressed by WalR PubMed
  • induced by vancomycin PubMed
  • strongly induced in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:
  • WalR: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 188 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: