AcoL

• Description: acetoin dehydrogenase E3 component (dihydrolipoamide dehydrogenase)
  
  

Gene name	acoL
Synonyms	yfjH
Essential	no
Product	acetoin dehydrogenase E3 component (dihydrolipoamide dehydrogenase)
Function	acetoin utilization
Gene expression levels in SubtiExpress: acoL
Interactions involving this protein in SubtInteract: AcoL
Metabolic function and regulation of this protein in SubtiPathways: acoL
MW, pI	48 kDa, 5.273
Gene length, protein length	1374 bp, 458 aa
Immediate neighbours	acoC, acoR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

AcoR regulon, CcpA regulon, SigL regulon

The gene

Basic information

• Locus tag: BSU08090

Database entries

• BsubCyc: BSU08090

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein N(6)-(dihydrolipoyl)lysine + NAD⁺ = protein N(6)-(lipoyl)lysine + NADH (according to Swiss-Prot)

• Protein family: class-I pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)

• Paralogous protein(s): LpdV, PdhD

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • (AcoA-AcoB)-AcoC-AcoL

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU08090

• Structure:

• UniProt: O34324

• KEGG entry: [3]

• E.C. number: 1.8.1.4

Expression and regulation

• Operon: acoA-acoB-acoC-acoL PubMed

• Expression browser: acoL PubMed

• Sigma factor: SigL PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by acetoin (AcoR) PubMed
  • induced under anaerobic conditions (Fnr) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • AcoR: transcription activation (interaction with SigL-containing RNA polymerase) PubMed
  • Fnr: indirect positive regulation via NarG-NarH-NarJ-NarI PubMed

• Additional information:
  • the mRNA is very stable (half-life > 15 min) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 290 PubMed

• • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 233 PubMed

• • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 555 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage