EpsB

• Description: extracellular polysaccharide synthesis, protein tyrosine kinase
  
  

Gene name	epsB
Synonyms	yveL
Essential	no
Product	protein tyrosine kinase
Function	biofilm formation
Gene expression levels in SubtiExpress: epsB
Interactions involving this protein in SubtInteract: EpsB
Regulation of this protein in SubtiPathways: epsB
MW, pI	24 kDa, 9.918
Gene length, protein length	681 bp, 227 aa
Immediate neighbours	epsC, epsA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, biofilm formation, phosphoproteins

This gene is a member of the following regulons

AbrB regulon, RemA regulon, SinR regulon

The gene

Basic information

• Locus tag: BSU34360

Database entries

• BsubCyc: BSU34360

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate (according to Swiss-Prot)

• Protein family: BY kinase, see the Bacterial Protein Tyrosine Kinase Database)

• Paralogous protein(s): PtkA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: autophosphorylated on Tyr-225 and Tyr-227 PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • EpsA-EpsB (based on TkmA-PtkA interaction)

• Localization:

Database entries

• BsubCyc: BSU34360

• Structure: 2VED (CapB, the homolog in Staphylococcus aureus) PubMed

• UniProt: P71051

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO PubMed

• Expression browser: epsB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by SinR PubMed

• Regulatory mechanism:
  • SinR: transcription anti-activation (prevents binding of RemA) PubMed
  • RemA: transcription activation PubMed
  • AbrB: transcription repression PubMed

• Additional information:
  • induction by sequestration of SinR by SinI or SlrA PubMed
  • the epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO operon is not expressed in a ymdB mutant PubMed
  • the amount of the mRNA is substantially decreased upon depletion of RNase Y (this is likely due to the increased stability of the sinR mRNA) PubMed

Biological materials

• Mutant:
  • GP1518 (aphA3) PubMed, available in Jörg Stülke's lab
  • GP1519 (epsA-epsB, aphA3) PubMed, available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH) PubMed, available in Jörg Stülke's lab

• Antibody:

• FLAG-tag construct: GP1541 epsB-FLAG 3x spc (based on pGP1331) available in Jörg Stülke's lab

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage