CitA

• Description: minor citrate synthase
  
  

Gene name	citA
Synonyms	citA1
Essential	no
Product	minor citrate synthase
Function	unknown
Gene expression levels in SubtiExpress: citA
Metabolic function and regulation of this protein in SubtiPathways: citA
MW, pI	40 kDa, 5.778
Gene length, protein length	1098 bp, 366 aa
Immediate neighbours	citR, yhdF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

poorly characterized/ putative enzymes, sporulation proteins

This gene is a member of the following regulons

CitR regulon

The gene

Basic information

• Locus tag: BSU09440

Database entries

• BsubCyc: BSU09440

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acetyl-CoA + H₂O + oxaloacetate = citrate + CoA (according to Swiss-Prot)

• Protein family: citrate synthase family (according to Swiss-Prot)

• Paralogous protein(s): MmgD, CitZ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU09440

• Structure: 2C6X

• UniProt: P39119

• KEGG entry: [3]

• E.C. number: 2.3.3.1

Expression and regulation

• Operon: citA-yhdF PubMed

• Expression browser: citA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism: CitR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • GP676 (erm), available in the Stülke lab
  • GP1282 (citA::cat), available in Jörg Stülke's lab
  • GP1753 (citR citA::aphA3), the kanamycin resistance cassette can be cut out by introducing the cre-rekombinase into the chromosom of B. subtilis, available in Jörg Stülke's lab

• Expression vector:
  • pGP2516 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• FLAG-tag construct:
  • GP1287 (spc, based on pGP1331), available in Jörg Stülke's lab

• Antibody:

References

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

S Jin, A L Sonenshein
Transcriptional regulation of Bacillus subtilis citrate synthase genes.
J Bacteriol: 1994, 176(15);4680-90
[PubMed:8045899] [WorldCat.org] [DOI] (P p)

S Jin, A L Sonenshein
Identification of two distinct Bacillus subtilis citrate synthase genes.
J Bacteriol: 1994, 176(15);4669-79
[PubMed:8045898] [WorldCat.org] [DOI] (P p)