YvyD

• Description: general stress protein, required for ribosome dimerization in the stationary phase, required for protection against paraquat stress
  
  

Gene name	yvyD
Synonyms	yviI
Essential	no
Product	unknown
Function	dimerization of ribosomes in the stationary phase, protection against paraquat stress
Gene expression levels in SubtiExpress: yvyD
MW, pI	21 kDa, 5.184
Gene length, protein length	567 bp, 189 aa
Immediate neighbours	secA, smiA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

general stress proteins (controlled by SigB), resistance against oxidative and electrophile stress, membrane proteins, phosphoproteins

This gene is a member of the following regulons

SigB regulon, SigH regulon

The gene

Basic information

• Locus tag: BSU35310

Phenotypes of a mutant

• the mutant is cold-sensitive
• delayed recovery from stationary phase and delayed germination

Database entries

• BsubCyc: BSU35310

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ribosomal protein S30Ae family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-6 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • YvyD-ribosome (for ribosome dimerization) PubMed
• Localization:
  • membrane protein

Database entries

• BsubCyc: BSU35310

• Structure:

• UniProt: P28368

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yvyD PubMed

• Expression browser: yvyD PubMed

• Sigma factor: SigB PubMed, SigH PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • induced by stress (SigB) PubMed
  • induced under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 2138 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 3361 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Publications on the homologous proteins from other bacteria

Pranav Puri, Thomas H Eckhardt, Linda E Franken, Fabrizia Fusetti, Marc C A Stuart, Egbert J Boekema, Oscar P Kuipers, Jan Kok, Bert Poolman
Lactococcus lactis YfiA is necessary and sufficient for ribosome dimerization.
Mol Microbiol: 2014, 91(2);394-407
[PubMed:24279750] [WorldCat.org] [DOI] (I p)

Yury S Polikanov, Gregor M Blaha, Thomas A Steitz
How hibernation factors RMF, HPF, and YfiA turn off protein synthesis.
Science: 2012, 336(6083);915-8
[PubMed:22605777] [WorldCat.org] [DOI] (I p)