SrfAA

• Description: surfactin synthetase / competence
  
  

Gene name	srfAA
Synonyms	comL
Essential	no
Product	surfactin synthetase / competence
Function	antibiotic synthesis
Gene expression levels in SubtiExpress: srfAA
MW, pI	401 kDa, 4.871
Gene length, protein length	10764 bp, 3588 aa
Immediate neighbours	hxlR, srfAB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

miscellaneous metabolic pathways, biosynthesis of antibacterial compounds, membrane proteins, phosphoproteins

This gene is a member of the following regulons

Abh regulon, CodY regulon, ComA regulon, PerR regulon, PhoP regulon, Spx regulon

The gene

Basic information

• Locus tag: BSU03480

Phenotypes of a mutant

• altered cell death pattern in colonies PubMed
• reduced swarming of srfAA mutant of B. subtilis NCIB3610 PubMed

Database entries

• BsubCyc: BSU03480

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-1006 PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization: membrane PubMed

Database entries

• BsubCyc: BSU03480

• Structure:

• UniProt: P27206

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: srfAA-srfAB-comS-srfAC-srfAD PubMed

• Expression browser: srfAA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • repressed during hydrogen peroxide stress (PerR) PubMed
  • expressed at high cell density (ComA) PubMed
  • induced under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription activation PubMed
  • CodY: transcription repression PubMed
  • ComA: transcription activation PubMed
  • PerR: transcription activation PubMed
  • Spx: transcription repression PubMed
  • Abh: transcription repressionPubMed

• Additional information:
  • A ncRNA is predicted between hxlR and srfAA PubMed
  • expressed in all cells in a population PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)