PolY1

• Description: translesion synthesis (TLS-) DNA polymerase Y1, important for stationary phase mutagenesis, promotes efficient replisome progression through lagging-strand genes, thereby reducing potentially detrimental breaks and single-stranded DNA at these loci
  
  

Gene name	polY1
Synonyms	yqjH
Essential	no
Product	translesion synthesis (TLS-) DNA polymerase Y1
Function	generation of mutations in stationary phase
Gene expression levels in SubtiExpress: polY1
Interactions involving this protein in SubtInteract: PolY1
Metabolic function and regulation of this protein in SubtiPathways: polY1
MW, pI	46 kDa, 9.953
Gene length, protein length	1242 bp, 414 aa
Immediate neighbours	gndA, mifM
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

The gene

Basic information

• Locus tag: BSU23870

Phenotypes of a mutant

reduced UV-induced mutagenesis PubMed

Database entries

• BsubCyc: BSU23870

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)

• Protein family: DNA polymerase type-Y family (according to Swiss-Prot)

• Paralogous protein(s): PolY2, UvrX, YozK-YobH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • PolY1-DnaN (required for untargeted mutagenesis) PubMed
  • PolA-PolY1-DnaN PubMed

• Localization: cytoplasm (homogeneous)

Database entries

• BsubCyc: BSU23870

• Structure:

• UniProt: P54545

• KEGG entry: [3]

• E.C. number: 2.7.7.7

Expression and regulation

• Operon: polY1-gndA (according to DBTBS)

• Expression browser: polY1 PubMed

• Sigma factor:

• Regulation: constitutively expressed PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP1111 (spc), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)