DacA

• Description: penicillin-binding protein 5, major D-alanyl-D-alanine carboxypeptidase
  
  

Gene name	dacA
Synonyms
Essential	no
Product	penicillin-binding protein 5, D-alanyl-D-alanine carboxypeptidase
Function	carboxypeptidase
Gene expression levels in SubtiExpress: dacA
MW, pI	48 kDa, 5.668
Gene length, protein length	1329 bp, 443 aa
Immediate neighbours	guaB, pdxS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, membrane proteins

The gene

Basic information

• Locus tag: BSU00100

Database entries

• BsubCyc: BSU00100

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala (according to Swiss-Prot)

• Protein family: peptidase S11 family (according to Swiss-Prot)

• Paralogous protein(s): DacF

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (according to Swiss-Prot), membrane associated PubMed

Database entries

• BsubCyc: BSU00100

• Structure:

• UniProt: P08750

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: dacA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 219 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2346 PubMed

Biological materials

• Mutant:
  • 1A742 ( dacA::cat), PubMed, available at BGSC
  • 1A743 ( dacA::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Elitza I Tocheva, Javier López-Garrido, H Velocity Hughes, Jennifer Fredlund, Erkin Kuru, Michael S Vannieuwenhze, Yves V Brun, Kit Pogliano, Grant J Jensen
Peptidoglycan transformations during Bacillus subtilis sporulation.
Mol Microbiol: 2013, 88(4);673-86
[PubMed:23531131] [WorldCat.org] [DOI] (I p)

Letal I Salzberg, Leagh Powell, Karsten Hokamp, Eric Botella, David Noone, Kevin M Devine
The WalRK (YycFG) and σ(I) RsgI regulators cooperate to control CwlO and LytE expression in exponentially growing and stressed Bacillus subtilis cells.
Mol Microbiol: 2013, 87(1);180-95
[PubMed:23199363] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

D L Popham, M E Gilmore, P Setlow
Roles of low-molecular-weight penicillin-binding proteins in Bacillus subtilis spore peptidoglycan synthesis and spore properties.
J Bacteriol: 1999, 181(1);126-32
[PubMed:9864321] [WorldCat.org] [DOI] (P p)